Fermenter Technical Parameters
| Parameter | Description |
| Total volume | 0.2L,0.5L,1L,2L,3L,5L,10L,20L,30L,40L etc. |
| Working volume | 50%~70% |
| Tank | Single glass vessel with stainless steel bottom. Lid made by stainless steel 316L with ports, condenser on the cover |
| Stirring system | Mechanical drive(GJ type) or Magnetic drive(GC type, bottom/top drive), 3 six-blade paddle and 1 foam breaker, each paddle height adjustable. Stir speed: 50~1000rpm/50-500rpm |
| Sterilization | Off-site sterilization (Autoclave) |
| Gas control | Control by rotor meter, air flow 1-2vvm. |
| Feeding | Automatically feeding by peristaltic pump |
| Anti-foam control | Automatically add antifoamer by peristaltic pump |
| Temperature control | Auto-control, electric heating range water source add 5℃ to 70℃ |
| PH control | PH 2.00-12.00±0.1, auto-control adding acid/base by peristaltic pump, pH sensor from Hamilton/Mettler, Switzerland. |
| DO control | 0-150±3% displaying precision 0.1%, DO sensor from Hamilton/Mettler, Switzerland. DO coupling with agitation, oxygen. |
| Sampling | Manual sampling |
| Control parameters | 10 or 15 inch touch screen , Siemens PLC with control parameters (stirring, pH, DO, temperature, feeding, alarm, gas etc.), data analysis, data record, data output, language(RU/EN/CN) and other customized functions |
| Optional configuration | Remote control, Mass flow meter(auto control gas), O2/N2/CO2, gas(CO2) exhaust analysis, Level sensor, Turbidity(OD) sensor, ORP sensor, weight system, Live cell sensor(Biomass), Printing, Siemens touch screen, etc., |
Fermenter Description
Bioreactor with single borosilicate glass vessel and stainless steel dish bottom, with the head plate of SS316L at bottom stainless steel jacket. It should have the temperature control via circulation of hot/cold water in the jacket through an inbuilt thermo circulator unit (working temperature range 8ºC-80ºC above coolant temperature). The system be complete to measure and control pH, DO, stirrer speed (25-500rpm), gas, foam and temperature.
Control system: 10in touch screen , Siemens PLC control system with control parameters (stirring, pH, DO, temperature, feeding, gas etc.), data analysis, data logging, data output and printing, overrun alarm, language switching and other functions.
Fermenter Application
Applied for culture of mammal and animal cells through batch feeding or continuous perfusion
The large-scale culture technology of animal cells (hybridoma cells, CHO cells, insect cels, etc.) and its bioreactor engineeringcan be widely used in research, development and industrial production of antibodies, recombinant protein drugs, viral vaccinesand other biotechnological products.
The large-scale culture technology of animal cells and its bioreactor system consisting ofsuch core technolgies as serum-free suspension culture technology, culture process and bioreactor design amplification andenhancement technology, large-scale high-density feeding and continuous perfusion culture technology, computer real-timemonitoring and control technology of culture process, large-scale and high-efficiency production technology of virus products,etc. have been established.
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